abstract：:Channels of the surface-connected, open canalicular system (OCS) of human platelets serve as the pathway for transport of substances into the cells and as conduits for the discharge of alpha granule products secreted during the platelet release reaction. The purpose of the present study was to determine if both functi...

journal_title：European journal of cell biology

authors： White JG,Escolar G

更新日期：1991-12-01 00:00:00

abstract：:When treated with an anti-kinetochore antibody present in the sera of scleroderma (var. CREST) patients, most chromosomes exhibit kinetochore dots at the position of the centromere. In this paper we report that some chromosomes in the mouse x human somatic cell hybrid fail to show these dots. In the early passages in ...

journal_title：European journal of cell biology

authors： Vig BK,Sternes K,Paweletz N

更新日期：1991-12-01 00:00:00

abstract：:We examined the secretion of three serum proteins, albumin (RSA), alpha 2 mu-globulin (alpha 2 mu G), and transferrin (Trf), in the isolated perfused liver. Within 4 h of perfusion, only 20 to 35% of previously synthesized proteins were secreted by the liver into the recirculating medium. Low temperature inhibited the...

journal_title：European journal of cell biology

authors： Lenk SE,Fisher DL,Dunn WA Jr

更新日期：1991-12-01 00:00:00

abstract：:ATP-dependent interactions between myosin and actin in the lower eukaryote, Physarum polycephalum, are inhibited by micromolar levels of Ca2+. This inhibition is mediated by the binding of Ca2+ to myosin, the phosphorylation of which is required if Ca2+ is to inhibit the activities of myosin (Kohama, K., Trends Pharma...

journal_title：European journal of cell biology

authors： Okagaki T,Ishikawa R,Kohama K

更新日期：1991-10-01 00:00:00

abstract：:Procaine-treated eggs can be penetrated by more than one spermatozoon. Supernumerary male pronuclei can fuse with the female one giving raise to multipolar spindles or remain isolated within the egg's cytoplasm forming their own spindle. In all types of multiple mitotic figures (asters and spindles) the distribution o...

journal_title：European journal of cell biology

authors： Czihak G,Kojima M,Linhart J,Vogel H

更新日期：1991-08-01 00:00:00

abstract：:D-amino acid oxidase is expressed to a high level in the yeast Rhodotorula gracilis (0.3% of total cell protein) through induction by D-alanine in a defined growth medium. Monospecific polyclonal antibodies against pure enzyme were obtained. Western blot analysis showed that the enzyme is synthesized as the mature pol...

journal_title：European journal of cell biology

authors： Perotti ME,Pollegioni L,Pilone MS

更新日期：1991-06-01 00:00:00

abstract：:Mitotic PtK1 spindles were UV irradiated (285 nm) during metaphase and anaphase between the chromosomes and the pole. The irradiation, a rectangle measuring 1.4 x 5 microns parallel to the metaphase plate, severed between 90 and 100% of spindle microtubules (MTs) in the irradiated region. Changes in organization of MT...

journal_title：European journal of cell biology

authors： Snyder JA,Armstrong L,Stonington OG,Spurck TP,Pickett-Heaps JD

更新日期：1991-06-01 00:00:00

abstract：:We have analyzed the mode of uptake of human beta FcRII molecules expressed in BHK cells (clone 2/14). When challenged with aggregated human IgG (ahIgG), these cells bind the ligand at 4 degrees C and endocytose the IgG: receptor complexes rapidly upon warming to 37 degrees C, as seen by fluorescence microscopy with a...

journal_title：European journal of cell biology

authors： Höning S,Jockusch BM,Kreimer G,Veltel D,Robenek H,Engelhardt W,Frey J

更新日期：1991-06-01 00:00:00

abstract：:A mitosis-specific centrosomal component was studied with a human autoantibody, SP-H, which immunostained mitotic poles and interphase nuclei, and a single polypeptide with an apparent molecular mass of 200 to 230 kDa in various lines of cultured cells. Early mitotic PtK1 cells treated with 10 micrograms/ml taxol cont...

journal_title：European journal of cell biology

authors： Maekawa T,Leslie R,Kuriyama R

更新日期：1991-04-01 00:00:00

abstract：:The interaction of daunomycin (DAU), an anthracyclinic antibiotic employed as antitumoral agent, with microtubules, has been investigated by cytochemical and morphological methods on a human melanoma cell line (H14). Results obtained indicated that DAU was able to modulate the microtubule reassembly in cells treated w...

journal_title：European journal of cell biology

authors： Molinari A,Calcabrini A,Crateri P,Arancia G

更新日期：1991-04-01 00:00:00

abstract：:The plasma membrane of nucleated erythrocytes contains a microtubular marginal band which appears to be associated with the plasma membrane skeleton. In this report, we identify two families of cytoskeletal proteins which may be involved in such an association. These proteins, of molecular mass 78 kDa and 48 kDa on SD...

journal_title：European journal of cell biology

authors： Stetzkowski-Marden F,Deprette C,Cassoly R

更新日期：1991-02-01 00:00:00

abstract：:We have measured the rate of secretion in vitro of individual rat exocrine pancreatic enzymes and proenzymes from cells in pancreatic lobules of control animals and animals subjected to 24 h optimal hormonal prestimulation with cerulein, in vivo. With the controls secreted proteins were first detectable at 20 to 25 mi...

journal_title：European journal of cell biology

authors： Vasiloudes P,Paul N,Tooze J,Kern HF

更新日期：1991-02-01 00:00:00

abstract：:The intracellular storage of zinc in Malpighian tubules of Drosophila hydei was studied by X-ray microanalysis of freeze-dried cryosections. Mass dense vacuoles in the proximal region of the anterior larval Malpighian tubule cells were found to accumulate zinc, not sodium. The zinc content was enhanced considerably af...

journal_title：European journal of cell biology

authors： Zierold K,Wessing A

更新日期：1990-12-01 00:00:00

abstract：:Crude protein extracts of Uromyces appendiculatus contain a polypeptide that resembles actin in several ways. This protein eluates from DEAE-cellulose with concentrations of KCl known to release actin of other species from the cation. The polypeptide is recognized by polyclonal antibodies directed to sodium dodecyl su...

journal_title：European journal of cell biology

authors： Tiburzy R,Hoch HC,Staples RC

更新日期：1990-12-01 00:00:00

abstract：:Appreciable amounts of glycosaminoglycans have been found by immunocytochemistry within mature elastin fibers of human dermis. On thin sections, elastin fibers showed antigenic sites for monoclonal antibodies recognizing the unsaturated units remaining after digestion of hyaluronic acid with Streptomyces hyaluronidase...

journal_title：European journal of cell biology

authors： Baccarani-Contri M,Vincenzi D,Cicchetti F,Mori G,Pasquali-Ronchetti I

更新日期：1990-12-01 00:00:00

abstract：:Monoclonal antibodies to the constitutive desmosomal glycoprotein desmoglein were characterized whose epitopes are located intracellularly, i.e., in the cytoplasmic portion of this molecule, and contribute to the structure of the desmosomal plaque. Using one of these antibodies (DG3.10), a peptide was isolated from a ...

journal_title：European journal of cell biology

authors： Koch PJ,Walsh MJ,Schmelz M,Goldschmidt MD,Zimbelmann R,Franke WW

更新日期：1990-10-01 00:00:00

abstract：:The subcellular distribution of a recently described neutral trimming mannosidase, the Man9-mannosidase, has been investigated by immunoelectron microscopy in pig hepatocytes. This enzyme processes asparagine-linked N-acetylglucosamine2 mannose9 oligosaccharides by specifically cleaving three of the four alpha 1,2-lin...

journal_title：European journal of cell biology

authors： Roth J,Brada D,Lackie PM,Schweden J,Bause E

更新日期：1990-10-01 00:00:00

abstract：:Actin and tubulins of Phytophthora infestans germlings were detected with monoclonal antibodies on Western blots of crude extracts separated by one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The Mr of actin was approximately 43,000, whereas alpha- and beta-tubulin, which migrated...

journal_title：European journal of cell biology

authors： Temperli E,Roos UP,Hohl HR

更新日期：1990-10-01 00:00:00

abstract：:A fodrin-like protein purified from porcine thyroid cells and characterized by its properties identical to those of pig brain spectrin (F. Regnouf et al., Eur. J. Biochem. 153, 313-319 (1985)) has been localized by immunofluorescence and electron immunocytochemistry in porcine and rat thyroid. Fodrin-like polypeptides...

journal_title：European journal of cell biology

authors： Gabrion JB,Barrière H,Nguyen Than Dao B,Chambard M,Mauchamp J,Regnouf F,Pradel LA

更新日期：1990-08-01 00:00:00

abstract：:Endogenous carbohydrate-binding sites were studied during rat cerebellar development on sections of fixed tissue using synthetic tools, biotinylated neoglycoproteins, in conjunction with subsequent avidinperoxidase staining. Neoglycoproteins were constructed by chemically coupling the histochemically pivotal carbohydr...

journal_title：European journal of cell biology

authors： Kuchler S,Zanetta JP,Vincendon G,Gabius HJ

更新日期：1990-06-01 00:00:00

abstract：:The lectins Griffonia simplicifolia I and Lycopersicon esculentum were used to assess the presence of endothelium-specific glycoproteins in the microvasculature of the rat myocardium, diaphragm and superficial cerebral cortex. Organs fixed by intravascular perfusion were processed to obtain semithin (0.5 micron) and t...

journal_title：European journal of cell biology

authors： Porter GA,Palade GE,Milici AJ

更新日期：1990-02-01 00:00:00

abstract：:We have reported previously that the human promyelocytic leukemia cell line HL-60, in its undifferentiated state, is devoid of cytoplasmic intermediate filament proteins and nuclear lamins A and C, but does express lamin B. Using immunofluorescence and immunoblotting techniques, we have further investigated the expres...

journal_title：European journal of cell biology

authors： Paulin-Levasseur M,Giese G,Scherbarth A,Traub P

更新日期：1989-12-01 00:00:00

abstract：:The distribution of microtubules and centrosomes was studied during sheep fertilization by electron and immunofluorescence microscopy. Tubulin and centrosomal material was identified with monoclonal anti-alpha-tubulin and MPM-2 antibodies, respectively. In ovulated eggs, microtubules were exclusively found in the meio...

journal_title：European journal of cell biology

authors： Le Guen P,Crozet N

更新日期：1989-04-01 00:00:00

abstract：:Evidence for the presence of a Cdc2-like protein in Physarum polycephalum has been obtained using a peptide antibody directed against a highly conserved amino acid sequence near the N-terminal end of Cdc2, Cdc28 and Cdc2HS. The antibody detected a 34 kDa cytoplasmic protein, similar in apparent size to Cdc2 in yeast a...

journal_title：European journal of cell biology

authors： Shipley GL,Sauer HW

更新日期：1989-02-01 00:00:00

abstract：:Zymogen granules (ZG) of rat pancreas have been isolated by the procedure of Pâquet et al. The granules lysed when exposed to alkaline pH (pH 8.2), and their membranes could be subfractionated by centrifugation on a sucrose gradient. Four discrete types of membranes corresponding to densities of 1.105, 1.085, 1.075, a...

journal_title：European journal of cell biology

authors： Beaudoin AR,Gilbert L,St-Jean P,Grondin G,Cabana C

更新日期：1988-12-01 00:00:00

abstract：:A great body of data increasingly point to the cell membrane as an important target for adriamycin (ADR). However, the exact mechanism by which ADR exerts its cytotoxic action through the interaction with the plasma membrane is still unknown. In this study, the interaction of ADR with red blood cells from healthy dono...

journal_title：European journal of cell biology

authors： Arancia G,Molinari A,Crateri P,Calcabrini A,Silvestri L,Isacchi G

更新日期：1988-12-01 00:00:00

abstract：:To investigate the association of calmodulin (CaM) with microtubules (MTs) in the mitotic apparatus (MA), the distributions of both CaM and tubulin were examined in mitotic PtK1 cells in which MT subclasses had been selectively removed or altered by treatment with cold or with the MT inhibitor, nocodazole. A fluoresce...

journal_title：European journal of cell biology

authors： Sweet SC,Welsh MJ

更新日期：1988-10-01 00:00:00

abstract：:Differences in the carbohydrate composition were found in the jellies and the cortex of the unfertilized Xenopus laevis egg using lectins and blood group antibodies. Blood group H trisaccharide was detected in the outer jelly and "A-like" oligosaccharide was found in the inner jelly. The blood group A trisaccharide wa...

journal_title：European journal of cell biology

authors： Tilley L,Dalix AM,Touitou H,Oriol R,Audit CO

更新日期：1988-10-01 00:00:00

abstract：:A novel cytochemical method for the in situ, ultrastructural localization of phospholipids in biological tissues is reported. The method is based on the enzyme-gold approach (M. Bendayan: J. Histochem. Cytochem. 29, 531, 1981). Phospholipase A2 from bee venom was adsorbed on colloidal gold particles (PLA2-gold) and ap...

journal_title：European journal of cell biology

authors： Coulombe PA,Kan FW,Bendayan M

更新日期：1988-08-01 00:00:00

abstract：:The nucleolar protein B-36 is an RNA-associated protein which has a number of properties in common with pre-mRNA-binding proteins (hnRNP proteins). Like the hnRNP proteins, B-36 appears to be evolutionarily conserved among various eukaryotes (protists and several animal species). The conservation of B-36 throughout th...

journal_title：European journal of cell biology

authors： Guiltinan MJ,Schelling ME,Ehtesham NZ,Thomas JC,Christensen ME

更新日期：1988-08-01 00:00:00

abstract：:When human blood platelets spread on a substratum they increase their surface area as much as 4-fold. We investigated the mechanism of spreading by light microscopy and by scanning and transmission electron microscopy. Contact of a platelet with a glass surface induces formation of thin extensions which spread out ove...

journal_title：European journal of cell biology

authors： Behnke O,Bray D

更新日期：1988-06-01 00:00:00

abstract：:We have used plant root tips frozen under high pressure in conjunction with freeze-fracture electron microscopy a) to evaluate the quality of freezing of unfixed, non-cryoprotected tissues obtainable with this method, b) to examine the structure of cells frozen under high pressure, c) to evaluate the usefulness of hig...

journal_title：European journal of cell biology

authors： Craig S,Staehelin LA

更新日期：1988-04-01 00:00:00

abstract：:The subcellular distribution of blood group A gene specified alpha 1,3N-acetylgalactosaminyltransferase and its product was studied in human intestinal goblet cells by immunoelectron microscopy. The O-glycosylation step yielding blood group A-active glycoconjugates occurred in the trans region of the Golgi apparatus a...

journal_title：European journal of cell biology

authors： Roth J,Greenwell P,Watkins WM

更新日期：1988-04-01 00:00:00

abstract：:Fibroblasts from I-cell disease, a genetically-determined lysosomal storage disease, are shown to contain large amounts of phase-dense lysosomes. These lysosomes accumulated acridine orange and were specifically labeled with antibodies to arylsulfatase A. In normal skin fibroblasts the number of arylsulfatase-containi...

journal_title：European journal of cell biology

authors： Waheed A,Van Etten RL,Koob R,Drenckhahn D

更新日期：1988-02-01 00:00:00

abstract：:Specific antibodies to a protein designated P1 (Mr approximately equal to 63,000), which is specifically altered in mutants resistant to the microtubule inhibitor podophyllotoxin, bind to mitochondria in cells of various vertebrate and invertebrate species (Eur. J. Cell Biol. 44, 278-285 (1987); Can. J. Biochem. Cell ...

journal_title：European journal of cell biology

authors： Gupta RS,Austin RC

更新日期：1987-12-01 00:00:00

abstract：:The major families of proteoglycans in human arterial tissue have been localized and characterized by electron microscopy. After staining with the polycationic dye cuprolinic blue in the presence of a critical electrolyte concentration, three differently sized populations of proteoglycan-cuprolinic blue precipitates a...

journal_title：European journal of cell biology

authors： Völker W,Schmidt A,Buddecke E

更新日期：1987-12-01 00:00:00

abstract：:Tight junctions (TJ) of the fascia occludens type can be rapidly assembled in the human colon adenocarcinoma cell line HT 29 under the influence of trypsin or ammonium sulfate. We have studied the influence of the metabolic inhibitors, dinitrophenol (DNP) and deoxyglucose (DG), on the induced formation of TJ in this c...

journal_title：European journal of cell biology

authors： Mückter H,Ben-Shaul Y,Bacher A

更新日期：1987-10-01 00:00:00

abstract：:The weak bases chloroquine, primaquine, NH4Cl and the ionophore monensin exert similar but not identical effects on sorting, transport and processing of cathepsin D in several human cell lines (fibroblasts, HepG2 cells, U937, monocytes). The drugs inhibit the segregation of newly synthesized cathepsin D from the secre...

journal_title：European journal of cell biology

authors： Braulke T,Geuze HJ,Slot JW,Hasilik A,von Figura K

更新日期：1987-06-01 00:00:00

abstract：:In order to investigate the relationship of different cytokeratins within one cell, monoclonal antibodies directed against three trophectoderm cytokeratins TROMA 1, 2 and 3 were microinjected into mouse teratocarcinoma-derived trophoblastoma cells and indirect immunofluorescence tests were used to follow the subsequen...

journal_title：European journal of cell biology

authors： Boller K,Kemler R,Baribault H,Doetschman T

更新日期：1987-06-01 00:00:00

abstract：:The importance of intact microtubules in the processing, storage and regulated secretion of von Willebrand factor (vWf) from Weibel-Palade bodies in endothelial cells was investigated. Human umbilical vein endothelial cells treated for 1 h with colchicine (10(-6) M) or nocodozole (10(-6) M) lost their organized microt...

journal_title：European journal of cell biology

authors： Sinha S,Wagner DD

更新日期：1987-06-01 00:00:00